As a prospective veterinarian, admitted Artina uneasy with the phenomenon of the spread of AI virus in Indonesia who have been eating quite a lot of casualties. He fully understands the risks of people who interact directly with the avian virus would be susceptible to this.
Faculty of Veterinary Medicine University of Gadjah Mada, Artina Prastiwi, find herbs that can inhibit the growth of the bird flu virus / avian influeza (AI) H5N1. The invention won first prize in the competition Scientists Society and Technology Indonesia (MITI) Paper Challenge (MPC) in 2011 which was held last January 29, 2011.
Artina found the Mahkota Dewa fruit extract (Phaleria macrocarpa) was effectively inhibited virus that had horrendous world a few years ago. Student class of 2007 won the first winner thanks to propose a series of studies contained in a scientific paper titled “Infusion Extracts Antiviral Activity Test Mahkota Dewa (Phaleria macrocarpa) In Ovo In Replication Inhibition of H5N1 virus vaccine Organic For Pioneer In Asia”. He won over 96 other papers from all over Indonesia.
He worried about the fate of the breeders who not only suffered material losses due to this deadly virus attacks, but also the safety of their souls. Although farmers know the risks that arise, they rarely give the vaccine to ward off the AI virus to poultry. “Many farmers do not vaccinate poultry because of the price of the vaccine chemical AI on the market is quite expensive. The price is set at 200 thousand dollars for 100 doses, “he quoted page UGM, Thursday, March 3, 2011.
Starting from the fact that this veiled girl trying to find solutions to address these troubling issues. He used Phaleria, local potentials Indonesia, which has been scientifically proven to increase endurance as antivirus AI. The content of saponin in Phaleria useful addition can improve the immune system and vitality can also be used as an anti-bacterial and anti-virus.
To get the compound saponin, Artina Phaleria extract through distillation. How to create antivirus from extracts of crown of God is preceded by weighing appropriate dose required. For a dose of 10 ml is needed Phaleria dry 100 grams per 100 ml of water or multiples of 100 grams per 1000 ml. “Next to get extract made distillery,” he said at GMU.
After obtaining the extract, Artina do saponin levels in laboratory testing LPPT UGM. He tested 10 ml saponin levels in LPPT UGM. According to him, extract the crown of the god must contain saponin content of 10 percent. The results obtained saponin is used as a raw material that is as a solvent suspense AI virus antigen. Then used as a vaccine is the crown of the god of 0.2 ml extract.
At first, spoken Artina, tests performed at 30 embryonated chicken eggs. From the test results are known egg that was given the AI virus and given an additional 10 percent of saponin extract Phaleria 0.2 ml were incubated for 35 days after embryo are not known to die, healthy and without a scar. While the eggs are injected with the higher dose 15 percent and 20 percent, it turns out all the embryos die with a form of bleeding throughout the body, dwarfism, and alantois turbid fluid.
“Ten percent is the best result to inhibit the bird flu virus. This proves that the levels of saponins which are used must be precise because it can cause poisoning when given in large doses, “he said.
Once proven safe in the egg, the couple’s daughter and Wartinah Sugita is testing this in chickens younger than 21 days and the results are quite encouraging. Chickens that have been vaccinated none of whom suffered
It said the girl born in Gunungkidul, January 26, 1989, developed a vaccine that proved capable of inhibiting the development of AI virus to 87 percent. In addition to proven in laboratory scale capable of inhibiting AI virus, the vaccine is also cheaper than chemical vaccines sold in the market.
AI vaccine on the market are usually priced 200 thousand rupiah per 100 dose. While vaccine-made 75 thousand dollars per 100 doses. Although highly effective and inexpensive, this vaccine has not been mass marketed. “It remains to be done further research, to know for certain results,” said alumnus SMA 1 Wonosari this.
The results of this study according to the plan will also presented in International seminar held in AMSTECS in Japan on 19-20 March.
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